Classical and Basal Cell Culture Media
Promocell GmbH PromoCell Endothelial Cell Basal Medium 500 ml
Endothelial Cell Growth Medium is a low-serum (2% V/V) medium optimized for the cultivation of endothelial cells from large blood vessels.Although all PromoCell media are optimized for use with primary human cells, we have received feedback from customers that this particular medium can also be used for bovine, porcine, murine, caprine, rabbit, elephant, dog, and rat endothelial cells from large vessels as well as for various endothelial cell lines.| Media supplement | Final supplement concentration || Fetal Calf Serum | 0.02 ml / ml || Endothelial Cell Growth Supplement | 0.004 ml / ml || Epidermal Growth Factor (recombinant human) | 0.1 ng / ml || Basic Fibroblast Growth Factor (recombinant human) | 1 ng / ml || Heparin | 90 μg / ml || Hydrocortisone | 1 μg / ml |Note: For best transfection results we recommend using our heparin-free Endothelial Cell Growth Medium MV2.
Promocell GmbH PromoCell Endothelial Cell Growth Medium 2 500 ml, ready-to-use, low serum, with Supplement Mix C-39216 attached to the flask
Endothelial Cell Growth Medium 2 is a low-serum (2% V/V) medium that lacks Endothelial Cell Growth Supplement (ECGS, bovine hypothalamic extract) but contains Insulin-like Growth Factor (Long R3 IGF) and Vascular Endothelial Growth Factor (VEGF). Because of its multiple effects on cell metabolism, VEGF may interfere with certain experimental setups. In these cases, we recommend the use of Endothelial Cell Growth Medium, which contains no VEGF.| Media supplement | Final supplement concentration || Fetal Calf Serum | 0.02 ml / ml || Epidermal Growth Factor (recombinant human) | 5 ng / ml || Basic Fibroblast Growth Factor (recombinant human) | 10 ng / ml || Insulin-like Growth Factor (Long R3 IGF) | 20 ng / ml || Vascular Endothelial Growth Factor 165 (recombinant human) | 0.5 ng / ml || Ascorbic Acid | 1 µg / ml || Heparin | 22.5 μg / ml || Hydrocortisone | 0.2 µg / ml |For best transfection results we recommend using our heparin-free Endothelial Cell Growth Medium MV2.
Promocell GmbH PromoCell Renal Epithelial Cell Growth Medium 2 KIT 500 ml, Contains: C-26230, C-39605
PromoCell Renal Epithelial Cell Growth Medium 2 is an optimized low-serum (5% V/V) medium that additionally contains Epinephrine. The combination of low serum and selected growth factors ensures successful growth in vitro making the Renal Epithelial Cell Growth Medium 2 the first choice for PromoCell Renal Epithelial Cells.Although all PromoCell media are optimized for use with primary human cells, we have received feedback from customers that this particular medium can also be used for porcine and mouse renal epithelial cells.| Media supplement | Final supplement concentration|| Fetal Calf Serum | 0.05 ml / ml || Epidermal Growth Factor (recombinant human) | 10 ng / ml || Insulin (recombinant human) | 5 μg / ml || Hydrocortisone | 36 ng / ml || Epinephrine | 0.5 μg / ml || Triiodo-L-thyronine | 4 pg / ml || Transferrin (recombinant human) | 5 μg / ml |
Promocell GmbH PromoCell Fibroblast Growth Medium 3 500 ml, ready-to-use, with C-39345 attached to the flask
PromoCell Fibroblast Growth Medium 3 has been developed for the in vitro cultivation of adult Human Cardiac Fibroblasts (HCF).Although all PromoCell media are optimized for use with primary human cells, we have received feedback from customers that this particular medium can also be used for porcine cardiac fibroblasts.| Media supplement | Final supplement concentration|| Fetal Calf Serum | 0.1 ml / ml || Basic Fibroblast Growth Factor (recombinant human) | 1 ng / ml || Insulin (recombinant human) | 5 μg / ml |
Promocell GmbH PromoCell Preadipocyte Differentiation Medium KIT 500 ml, Contains: C-27411, C-39437
The Preadipocyte Differentiation Medium is a defined and animal-component free medium, which induces the differentiation of preadipocytes into mature adipocytes. The mature adipocytes can be cultivated using the Adipocyte Nutrition Medium.Although all PromoCell media are optimized for use with primary human cells, we have received feedback from customers that this particular medium can also be used for murine preadipocytes/adipocytes.Components:* Differentiation Medium (Ready-to-use): Includes Basal Medium and SupplementMix* Differentiation Medium Kit: Includes Basal Medium and SupplementPack* SupplementMix: Contains all media supplements premixed in one vial* SupplementPack: Contains all media supplements as individual vials| Media supplement | Final supplement concentration || d-Biotin | 8 μg / ml || Insulin (recombinant human) | 0.5 μg / ml || Dexamethasone | 400 ng / ml || IBMX | 44 μg / ml || L-Thyroxine | 9 ng / ml || Ciglitazone | 3 μg / ml |
Promocell GmbH PromoCell Fibroblast Basal Medium 3 500 ml
PromoCell Fibroblast Growth Medium 3 has been developed for the in vitro cultivation of adult Human Cardiac Fibroblasts (HCF).Although all PromoCell media are optimized for use with primary human cells, we have received feedback from customers that this particular medium can also be used for porcine cardiac fibroblasts.Components:* Growth Medium (Ready-to-use): Includes Basal Medium and SupplementMix* Growth Medium Kit: Includes Basal Medium and SupplementPack* SupplementMix: Contains all media supplements premixed in one vial* SupplementPack: Contains all media supplements as individual vials| Media supplement | Final supplement concentration|| Fetal Calf Serum | 0.1 ml / ml || Basic Fibroblast Growth Factor (recombinant human) | 1 ng / ml || Insulin (recombinant human) | 5 μg / ml |
Promocell GmbH PromoCell Fibroblast Basal Medium 2 500 ml
PromoCell Fibroblast Growth Medium 2 is a low-serum (2% V/V) medium and has been developed for the in vitro cultivation of adult Human Fibroblasts.Although all PromoCell media are optimized for use with primary human cells, we have received feedback from customers that this particular medium can also be used for bovine, porcine, and rat fibroblasts, as well as fibroblast cell
Promocell GmbH PromoCell Osteoblast Mineralization Medium 100 ml, ready-to-use, with C-39616 attached to the flask
PromoCell Osteoblast Mineralization Medium has been developed for the efficient induction of strong mineralization in human osteoblast cultures. Extensive extracellular calcium-deposits can be observed after 21 days by following an easy one-step protocol.Although all PromoCell media are optimized for use with primary human cells, we have received feedback from customers that this particular medium can also be used for bovine, murine, and rat osteoblasts as well as osteoblast cell lines.To view a list of references where this medium was used with other species/cell types please check the reference literature tab on the manufacturerer homepage.
Promocell GmbH PromoCell Supplement Pack / Foll. Derm. Papilla Cell Growth Medium Contains: C-37330, C-30310, C-31010, C-30021
PromoCell Follicle Dermal Papilla Cell Growth Medium is a low-serum medium that has been developed for the in vitro cultivation of Human Follicle Dermal Papilla Cells.The medium is optimized for primary cultures of fetal, neonatal, and adult tissue, or for the maintenance of Follicle Dermal Papilla Cells.Components:* Growth Medium (Ready-to-use): Includes Basal Medium and SupplementMix* Growth Medium Kit: Includes Basal Medium and SupplementPack* SupplementMix: Contains all media supplements premixed in one vial* SupplementPack: Contains all media supplements as individual vials| Media supplement | Final supplement concentration|| Fetal Calf Serum | 0.04 ml / ml || Bovine Pituitary Extract | 0.004 ml / ml || Basic Fibroblast Growth Factor (recombinant human) | 1 ng / ml || Insulin (recombinant human) | 5 μg / ml |
Promocell GmbH PromoCell Supplement Mix / Endothelial Cell Growth Medium MV 2
Endothelial Cell Growth Medium MV2 is a low-serum (5% V/V) medium developed for the in vitro cultivation of endothelial cells from microvascular vessels, the coronary artery, and the aorta.The medium lacks the Endothelial Cell Growth Supplement (ECGS, bovine hypothalamic extract) but contains basic Fibroblast Growth Factor, Insulin-like Growth Factor and Vascular Endothelial Growth Factor (VEGF). Because of its effects on cell metabolism, VEGF may interfere with certain experimental setups. In these cases, we recommend the use of Endothelial Cell Growth Medium MV.| Media supplement | Final supplement concentration || Fetal Calf Serum | 0.05 ml / ml || Epidermal Growth Factor (recombinant human) | 5 ng / ml || Basic Fibroblast Growth Factor (recombinant human) | 10 ng / ml || Insulin-like Growth Factor (Long R3 IGF) | 20 ng / ml || Vascular Endothelial Growth Factor 165 (recombinant human) | 0.5 ng / ml || Ascorbic Acid | 1 μg / ml || Hydrocortisone | 0.2 μg / ml |
Promocell GmbH PromoCell Supplement Mix / Airway Epithelial Cell Growth Medium
PromoCell Airway Epithelial Cell Growth Medium is a serum-free medium optimized for the in vitro cultivation of epithelial cells from large air passages.Although all PromoCell media are optimized for use with primary human cells, we have received feedback from customers that this particular medium can also be used for equine, porcine, murine, bat and rat airway epithelial cells.To view a list of references where this medium was used with other species/cell types please check manufacturer homepage.| Media supplement | Final supplement concentration|| Bovine Pituitary Extract | 0.004 ml / ml || Epidermal Growth Factor (recombinant human) | 10 ng / ml || Insulin (recombinant human) | 5 μg / ml || Hydrocortisone | 0.5 μg / ml || Epinephrine | 0.5 μg / ml || Triiodo-L-thyronine | 6.7 ng / ml || Transferrin (recombinant human) | 10 μg / ml || Retinoic Acid | 0.1 ng / ml |
Promocell GmbH PromoCell Supplement Mix / Fibroblast Growth Medium 3
PromoCell Fibroblast Growth Medium 3 has been developed for the in vitro cultivation of adult Human Cardiac Fibroblasts (HCF).Although all PromoCell media are optimized for use with primary human cells, we have received feedback from customers that this particular medium can also be used for porcine cardiac fibroblasts.To view a list of references where this medium was used with other species/cell types please check manufacturer homepage.Components:* Growth Medium (Ready-to-use): Includes Basal Medium and SupplementMix* Growth Medium Kit: Includes Basal Medium and SupplementPack* SupplementMix: Contains all media supplements premixed in one vial* SupplementPack: Contains all media supplements as individual vials| Media supplement | Final supplement concentration|| Fetal Calf Serum | 0.1 ml / ml || Basic Fibroblast Growth Factor (recombinant human) | 1 ng / ml || Insulin (recombinant human) | 5 μg / ml |
Promocell GmbH PromoCell Supplement Mix / Endothelial Cell Growth Medium MV
Endothelial Cell Growth Medium MV is a low-serum (5% V/V) medium developed for the in vitro cultivation of endothelial cells from microvascular vessels, the coronary artery, and the aorta.Although all PromoCell media are optimized for use with primary human cells, we have received feedback from customers that this particular medium can also be used for bovine, porcine, murine, rat and dog endothelial cells from microvascular vessels as well as for various endothelial cell lines.| Media supplement | Final supplement concentration || Fetal Calf Serum | 0.05 ml / ml || Endothelial Cell Growth Supplement | 0.004 ml / ml || Epidermal Growth Factor (recombinant human) | 10 ng / ml || Heparin | 90 µg / ml || Hydrocortisone | 1 µg / ml |Note: For best transfection results we recommend using our heparin-free Endothelial Cell Growth Medium MV2. Heparin, included in all our other Endothelial Cell Growth Media formulations, may reduce transfection efficiency.
Sigma Aldrich Fine Chemicals Biosciences Dulbeccos Modified Eagles
DULBECCOS Dulbeccos Modified Eagles